Andrology Laboratory Fellowship

Andrology Laboratory Fellowship is organized by MasterHealthPro Inc.

Description:
This course helps evaluate semen and its properties and elaborate on semen preparation for various ART procedures. The module delivered by experts includes lessons on all laboratory and Andrology protocols including Semen Analysis, Sperm Preparation Techniques, Semen Freezing, Advanced Sperm Function tests (Fructose, HOS, Vitality, Peroxidase Staining), Sperm DNA Fragmentation, Semen Banking & Sperm Retrieval Techniques. The module involves an ample number of video sessions for the audience to familiarize themselves with laboratory routines

Learning Objectives:

• Learn semen analysis and ancillary tests by the WHO sixth edition criteria
• Learn advanced sperm preparation methods used in assisted reproduction such as: IUI, IVF and ICSI
• Understand advanced sperm function tests
• Learn methods for male fertility preservation for use in ART

Learning Outcomes:

• Theoretical and technical knowledge of the clinical Andrology
• Knowledge of setting up Andrology lab
• Operational and Quality Management skills

Course Content:
Physiology & Anatomy

• Spermatogenesis and Spermiogenesis

Setting up of IUI Lab

• Setting Up of Andrology Laboratory
  • 1. Requirements
  • 2. Guidelines to setup IUI Lab

Lab Maintenance

• Quality Analysis and Quality Check
  • 1. Media and Disposables Handling
  • 2. Handling of Liquid Nitrogen
  • 3. LN2 Tank Maintenance
  • 4. Handling of Sero Positive Samples

Semen Analysis

• Macro Evaluation, Micro Analysis & DFI by Flow Cytrometry

Sperm Processing Techniques

• Sperm Preparation Techniques and Practical Implications
  • 1. Simple Wash
  • 2. Swim Up
  • 3. Double Density Gradient
  • 4. Microfluidics
  • 5. Retrograde Ejaculation Sample Preparation
  • 6. TESA Sperm processing Technique

Sperm Freezing Techiques

• Sperm Cryopreservation and Sperm Vitrification with fundamentals of cryobiology and implications to practice
  • 1. Slow Freezing Methods
  • 2. Vitrification
  • 3. TESA Sample Freezing

Upskilling

• CASA
• MACS & U Select Method